The Rv3874-Rv3875 chimeric protein shows a promiscuous serodiagnostic potential for tuberculosis.

Tuberculosis (TB) stays a major cause of death globally after COVID-19 and HIV. An early diagnosis to control TB effectively, needs a fast reliable diagnostic method with high sensitivity. Serodiagnosis involving polyclonal antibodies detection against an antigen of Mycobacterium tuberculosis (Mtb) in serum samples can be instrumental. In our study, Rv3874 and Rv3875 antigens were cloned, expressed, and purified individually and as a chimeric construct in Escherichia coli BL21. Enzyme-Linked Immunosorbent Assay (ELISA) based findings revealed that the Rv3874-Rv3875 chimeric construct was two-fold more sensitive (59.7%) than the individual sensitivities of Rv3874 (28.4%) and Rv3875 (24.9%) for 201 serum TB positive samples. Furthermore, the fusion construct was a little more sensitive (60.4%) for male subjects than that for females (58.8%). Lastly, our preliminary findings, molecular insights of secondary structure, and statistical and in silico analysis of each construct also advocate that CEP can be considered a better immunodiagnostic tool in addition to previously reported EC skin test.

Immunity debt or vaccination crisis? A multi-method evidence on vaccine acceptance and media framing for emerging COVID-19 variants.

Renewed COVID-19 outbreaks, stemming from the highly infectious Delta and Omicron variants, prompted rising fears of a 'pandemic among the unvaccinated'. To address this prevalent vaccination crisis, media framing communication strategies can amplify the scientific evidence on COVID-19 vaccines to reach diverse geographic and socio-economic communities. The critical role of media framing strategies to engage and encourage large populations regarding vaccine acceptance has been rarely studied, despite growing evidence on vaccine hesitancy. The present study used a multi-method approach (i.e., content analysis and quasi-experiments) that unpacked the framing practices employed by the mainstream media in Pakistan. The findings of the content analysis revealed that the media extensively used uncertainty, conflict, consequences, and action rather than new evidence and reassurance frames in its COVID-19 related campaigns. In a series of quasi-experiments involving 720 participants, we manipulated these six frames of COVID-19 related news coverage (i.e., uncertainty, conflict, consequences, action, new evidence, and reassurance) to investigate the underlying mechanism that influences vaccine acceptance. The findings established that the message-consistent effects of media frames manifesting fear (e.g., consequence and uncertainty) and action cues made receivers more supportive of vaccination. The present study findings theoretically address the calls for a more inclusive "community-health reporting model", besides offering new evidence on the media framing strategies to deliver more targeted, meaningful, and effective campaigns to raise public acceptance for COVID-19 vaccines.

Transhumant pastoralist knowledge of infectious diseases and adoption of alternative land use strategies in the Hindu-Kush Himalayan (HKH) region of Pakistan

Pastoralists’ awareness of infectious disease outbreaks depends on their socioeconomic characteristics and considerably affects their land use strategies. This study aimed to identify the factors that affect transhumant communities’ awareness of recently emerged Coronavirus Disease 2019 (COVID-19) in humans and foot and mouth disease (FMD) in animals. This study also investigated the key factors that govern the adoption of alternative land use strategies by nonmigrating transhumants to avoid seasonal migration in the Hindu-Kush Himalayan (HKH) region of Pakistan. To link the awareness of disease spread to the adoption of alternative land use strategies, a focus group discussion and a socioeconomic survey were conducted in the study area. A total of 300 households were randomly selected from a nonmigrating transhumant community. The results of the binary logit model showed that landholding size, good relationships with extension field staff, and extension contact were positively related, whereas household education was negatively related to pastoralists’ awareness of infectious disease outbreaks. In the case of adopting alternative strategies to avoid future long-distance migration, our model showed that pastoralists’ awareness of infectious disease outbreaks significantly and positively affected pastoralists’ adoption of these strategies. The other variables that were positively associated with the pastoralists’ adoption of these strategies included good relationships with extension field staff, household size and primary dependence on pastoral-based livelihood, whereas the education level of the head of the household, lack of financial resources and disputes over agricultural lands/forests were negatively associated with the pastoralists’ adoption of these strategies. We conclude that to avoid infectious disease spread and promote adoption of alternative strategies, the government should pay attention to creating awareness among transhumant communities. Communicating the latest scientific research on infectious disease outbreaks and effective control measures that are translated in an understandable (pictographic) manner could be an effective policy intervention.

Plant-Based Drugs and Vaccines for COVID-19.

The coronavirus SARS-CoV-2 has turned our own health and the world economy upside down. While several vaccine candidates are currently under development, antivirals with the potential to limit virus transmission or block infection are also being explored. Plant production platforms are being used to generate vaccines and antiviral proteins inexpensively and at mass scale. The following review discusses the biology and origins of the current coronavirus pandemic, and describes some of the conventional, synthetic, and plant-based approaches to address the challenge that it presents to our way of life.